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subcloning in pet14b  (Addgene inc)


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    Structured Review

    Addgene inc subcloning in pet14b
    Subcloning In Pet14b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet14b+plasmid/pSBtet-GP+(Plasmid+%2360495)/pmc11352870-27-12-29
    Average 93 stars, based on 46 article reviews
    subcloning in pet14b - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    Amplification:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    Clone Assay:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    Derivative Assay:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Expressing:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    Plasmid Preparation:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    Purification:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..

    In Vitro:

    Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes
    Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the pET14b plasmid (Addgene) for Escherichia coli expression and purification as in vitro TOR kinase substrates. ..

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation
    Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the pet14b plasmid (Addgene) for E. coli expression and purification as in vitro TOR kinase or S6K1 kinase substrates. ..



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    Addgene inc pet14b e coli exonuclease
    ( A ) Domain structure of E. coli SSB protein. ( B ) Structure of E. coli single-stranded-DNA binding protein as a tetramer (PDB structure 4MZ9). The IDP regions are red lines, and the acidic tips are shown as amino acid sequences. ( C ) SSB-Ct sequence and its numbering as a segment of the whole protein. wtSSB-Ct is an octapeptide with a sequence identical to the SSB-Ct segment. Sequences are colored according to the conservation scores (see lower part of panel ( C ), * denotes the highest score) obtained from PRALINE .
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    Image Search Results


    ( A ) Domain structure of E. coli SSB protein. ( B ) Structure of E. coli single-stranded-DNA binding protein as a tetramer (PDB structure 4MZ9). The IDP regions are red lines, and the acidic tips are shown as amino acid sequences. ( C ) SSB-Ct sequence and its numbering as a segment of the whole protein. wtSSB-Ct is an octapeptide with a sequence identical to the SSB-Ct segment. Sequences are colored according to the conservation scores (see lower part of panel ( C ), * denotes the highest score) obtained from PRALINE .

    Journal: Pharmaceutics

    Article Title: Structural Adaptation of the Single-Stranded DNA-Binding Protein C-Terminal to DNA Metabolizing Partners Guides Inhibitor Design

    doi: 10.3390/pharmaceutics15041032

    Figure Lengend Snippet: ( A ) Domain structure of E. coli SSB protein. ( B ) Structure of E. coli single-stranded-DNA binding protein as a tetramer (PDB structure 4MZ9). The IDP regions are red lines, and the acidic tips are shown as amino acid sequences. ( C ) SSB-Ct sequence and its numbering as a segment of the whole protein. wtSSB-Ct is an octapeptide with a sequence identical to the SSB-Ct segment. Sequences are colored according to the conservation scores (see lower part of panel ( C ), * denotes the highest score) obtained from PRALINE .

    Article Snippet: pET14b— E. coli Exonuclease I was a gift from Charles Bell (Addgene plasmid # 104552; http://n2t.net/addgene:104552 ; RRID: Addgene_104552, accessed on 23 July 2019).

    Techniques: Binding Assay, Sequencing