subcloning in pet14b (Addgene inc)
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Subcloning In Pet14b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet14b+plasmid/pSBtet-GP+(Plasmid+%2360495)/pmc11352870-27-12-29
Average 93 stars, based on 46 article reviews
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Construct:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the Amplification:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the Clone Assay:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the Derivative Assay:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Expressing:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the Plasmid Preparation:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the Purification:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the In Vitro:Article Title: Differential TOR activation and cell proliferation in Arabidopsis root and shoot apexes Article Snippet: .. For E2fb and ROP2 constructs, the coding regions were amplified from Arabidopsis Col-0 cDNA and then fused to the HA-tag and cloned between the 35S -derived promoter and the NOS terminator ( 22 ) for protoplast transient expression or fused to the 6×HIS tag in the Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation Article Snippet: The construct of 4E-BP1-6HIS was from Addgene. .. For constructs of E2fa (At2g36010) and its truncated variants (Δ1, amino acids 1-420; Δ2, amino acids 162-420; Δ3, amino acids 162-485; Δ4, amino acids 81-420; e2fa, amino acids 1-323) or RPS6B (amino acids 150-249), the coding regions were amplified from Arabidopsis Col-0 complementary DNA, then fused to the HA tag and cloned between the 35S-drived promoter and NOS terminator11,12 for protoplast transient expression, or fused to the 6HIS tag in the |
