Journal: Pharmaceutics
Article Title: Structural Adaptation of the Single-Stranded DNA-Binding Protein C-Terminal to DNA Metabolizing Partners Guides Inhibitor Design
doi: 10.3390/pharmaceutics15041032
Figure Lengend Snippet: ( A ) Domain structure of E. coli SSB protein. ( B ) Structure of E. coli single-stranded-DNA binding protein as a tetramer (PDB structure 4MZ9). The IDP regions are red lines, and the acidic tips are shown as amino acid sequences. ( C ) SSB-Ct sequence and its numbering as a segment of the whole protein. wtSSB-Ct is an octapeptide with a sequence identical to the SSB-Ct segment. Sequences are colored according to the conservation scores (see lower part of panel ( C ), * denotes the highest score) obtained from PRALINE .
Article Snippet: pET14b— E. coli Exonuclease I was a gift from Charles Bell (Addgene plasmid # 104552; http://n2t.net/addgene:104552 ; RRID: Addgene_104552, accessed on 23 July 2019).
Techniques: Binding Assay, Sequencing